Our research interests are focused on human skeletal muscle metabolism in response to exercise and nutrition, how this adapts with exercise training, physical inactivity and ageing, and how we can affect adaptation and performance with novel nutritional, exercise or pharmacological interventions.
We measure metabolism as directly as possible with stable isotope approaches, arterio-venous balance methods and/or muscle biopsy sampling (e.g. to determine acute and chronic muscle protein synthesis rates).
Our facilities: Nutritional Physiology Research Unit
We perform this research in the newly refurbished access controlled Nutritional Physiology Research Unit, which consists of:
Exercise physiology laboratories
Two exercise physiology laboratories each with a lode excaliber cycle ergometer, cortex metabolic cart, and capability for blood and muscle biopsy sampling, intravenous infusion of nutrients and stable isotopes of amino acids, fatty acids, and glucose.
Resting metabolism laboratories
Two single and one double resting metabolism laboratories with doppler ultrasound, cortex metabolic cart, and capability for bed rest and limb immobilization studies, nutritional interventions, blood and muscle biopsy sampling, arteriovenous forearm balance, and intravenous infusion of nutrients and stable isotopes of amino acids, fatty acids, and glucose.
State of the art gym with power rack, free weights, unilateral leg press, biodex dynamometer, 3 atom watt bikes, eccentric ergometer, and capability for various exercise training protocols.
Food preparation facilities
- A metabolic kitchen and food storage for preparation of acute or prolonged dietary/supplement interventions and 2H2O and/or H218O methods.
- -80 freezer room for sample storage.
We also have access to DXA, 1.5T MRI (soon to be upgraded to 3T), and a clinical research facility for the supply and administration of drugs such as insulin for glucose clamping, and research studies in patients.
We are also interested in the mechanisms underpinning adaptation and so our analytical facility and expertise allows for methods including low and high density RT-PCR arrays for muscle gene expression, western blot for protein content/phosphorylation status, HPLC, spectrophotometric and radioenzymatic assays of muscle metabolites and enzyme activities, fluorescence and electron microscopy, and mass spectrometry/isotope ratio of stable isotopes.